Clinical and histological evidence indicate that regeneration of periodontal tissues can be achieved. The aim is to show how an equine collagen membrane and an equine bone replacement graft can be successfully utilized for the regeneration of periodontal structures on questionable teeth. Two teeth, 13 and 35, in two patients with advanced intrabony defects, were treated by a regenerative periodontal therapy. Tooth 13 had an initial probing depth (PD) of 16 mm mesially while tooth 35 had a PD of 11 mm distally and 7 mm buccally together with pulp necrosis and class II mobility enhanced by occlusal trauma. After initial periodontal therapy, endodontic treatment and removal of occlusal disturbances, reevaluation shows PD reduction and remaining deep intrabony defects. Regenerative surgical therapy was applied on remaining intrabony defects: placement of an equine bone replacement graft (BIO-GEN MIX_, BIOTECK, Torino, Italy) and an equine membrane (BIOCOLLAGEN_, BIOTECK, Torino, Italy). At reevaluation 8 months after treatment, tooth 13 showed a PD reduction from preoperative 9 mm to 4 mm with an increase in recession of 1 mm while tooth 35 showed a PD reduction from 8 mm to 4 mm distally and from 4 mm to 2 mm buccally with no increase in recession. Control X-rays indicated an almost complete resolution of the intrabony defects. Successful restitution of the intrabony defects on questionable teeth can be achieved by combining an equine membrane and an equine bone replacement graft.
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