Logo Bioteck Academy

Ricerca Scientifica

INTERACTION OF OSTEOGENIN, A HEPARIN BINDING BONE MORPHOGENETIC PROTEIN, WITH TYPE IV COLLAGEN

1990
Iscriviti alla
Newsletter
Autore : Reddi A.H., Pointer R.H., Paralkar V.M., Nandedkar A.K., Kleinman H.K.

Osteogenin, an extracellular matrix component of bone, is a heparin binding differentiation factor that initiates endochondral bone formation in rats when implanted subcutaneously with an insoluble collagenous matrix. We have examined the interaction of Osteogenin with various extracellular matrix components including basement membranes.

Osteogenin, purified from bovine bone, binds avidly to type IV collagen and to a lesser extent to both type I and IX collagens. Osteogenin binds equally well to both native and denatured type IV collagen. Both alpha 1 and alpha 2 chains of type IV collagen are recognized by Osteogenin. Osteogenin binds to a collagen IV affinity column, and is eluted by 6.0 M urea with 1 M NaCl, pH 7.4, and the eluate contained the osteogenic activity as demonstrated in vivo. Binding of osteogenin to collagen IV is not influenced by either laminin or fibronectin. These results imply that Osteogenin binding to extracellular matrix components including collagens I and IV and heparin may have physiological relevance, and such interactions may modulate its local action.
The Journal of Biological Chemistry

Iscriviti alla Newsletter

  • Hidden
Logo Bioteck Academy


Vicenza
Sede Amministrativa e legale
via E. Fermi, 49
36057 Arcugnano, Vicenza (VI) - Italia
Tel. +39 0444 289366
Fax: +39 0444 285272


Contattaci


Torino
Centro Polifunzionale di produzione
Via G. Agnelli, 3
10020 Riva presso Chieri, Torino (TO) - Italia

Copyright © 2024 Bioteck S.p.A. - Privacy Policy - Cookie Policy