Osteoplant is an equine cortical and spongy bone tissue, used to fill bone defects in orthopedic, maxillofacial and dental surgery. To study the mechanism by which Osteoplant induces the reabsorption and the substitution of the graft with new bone, the expression of genes related to the osteoblast differentiation were analyzed using cultures of stem cells derived from dental pulp stem cells (ADSCs) treated with this biomaterial. The up-regulation of bone related genes like SPl, FOSLI, RUNX2 and SPPI and the disappearance of the mesenchymal stem cell marker ENG demonstrated that this biomaterial is conducive osteoblast and osteoclast. In particular the up-regulation of SPP1 demonstrated that this bone substitute material was actively resorbed by human osteoclasts. Our results confirmed that Osteoplant has high potential for use as bone tissue scaffold in bone tissue engineering.