Anorganic bovine bone has been well studied and proved to be effective for socket preservation because of its slow resorption over time. Non-antigenic, enzyme-treated equine bone has emerged as a possible alternative biomaterial and its effectiveness investigated in different bone regeneration indications, but at present no evidence exists about its use . The objective of this study is to report a first case about the use of a Freeze-dried bone paste of equine origin cylinder in socket regeneration and preservation, and present a first clinical and histological assessment. A variant of the enzyme-treated equine bone, featuring partially denatured bone collagen, and added with exogenous collagen to create a lyophilized dry paste, was used to graft a post-extractive socket in a patient needed tooth 34 to be extracted. No protective membrane was placed, and no specific flap preparation was performed. After 3 months, an osseointegrated implant was placed at the grafted site and a bone biopsy was collected for histologic and morphometric assessment. The patient healed uneventfully and was rehabilitated successfully. Socket bone levels were maintained on a short-term basis. Histological analysis of the bone biopsy showed that the graft material had undergone nearly complete remodelling with no signs of inflammation. Newly formed bone, residual biomaterial and medullar spaces were, respectively, 36.4%; 12.1% and 51.5%. The lyophilized equine bone paste allowed for successful short-term socket preservation and for effective bone regeneration. The handling properties of the graft were quite satisfactory. Studies in a controlled clinical setting aimed to investigate its effectiveness are highly advisable.