Cell-matrix interaction in bone: type I collagen modulates signal transduction in osteoblast-like cells

Propiedades de colágeno del hueso

1995

Cell interaction with extracellular matrix (ECM) modulates cell growth and differentiation

By using in vitro culture systems, we tested the effect of type I collagen (Coll-I) on signal transduction mechanisms in the osteosarcoma cell line UMR-106 and in primary cultures from neonatal rat calvariae. Cells were cultured for 72 h on Coll-I gel matrix and compared with control cells plated on plastic surfaces. Agonist-dependent and voltage-dependent rises in cytosolic Ca2+ concentration ([Ca2+]i; measured by fura 2 fluorometry) were significantly blunted in cells cultured on Coll-I compared with cells grown on plastic. In UMR-106 cells, the collagen matrix effect was mimicked by 24-h incubation with soluble Coll-I or short peptides containing the arginine-glycine-aspartate motif. Accumulation of cellular adenosine 3',5'-cyclic monophosphate (cAMP) stimulated by parathyroid hormone, cholera toxin, and forskolin was augmented (50-150%) in cells plated on Coll-I vs. control. The collagen effect on both [Ca2+]i- and adenylate cyclasesignaling pathways in UMR-106 cells was abrogated in the presence of protein kinase C (PKC) depletion or inhibition.
Also, Coll-I induced a twofold increase in membrane-bound PKC without changing cytosolic PKC activity. Thus, by altering PKC activity, Coll-I modulates the [Ca2+]i- and cAMP-signaling pathways in osteoblasts. This, in turn, may influence bone remodeling processes.

Publicado en American Journal of Physiology

Pubmed

ALTRE PUBBLICAZIONI

The size exclusion characteristics of type I collagen

Effect of type I collagen on the adhesion, proliferation, and osteoblastic gene expression of bone marrow derived mesenchymal stem cells

The effect on osteogenesis of type I collagen applied to experimental bone defects

Pubmed